PhD Program in Montepllier

 

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De : Didier MARGUET
Envoyé : vendredi 13 mars 2009 13:59
À : labs
Objet : TR: PhD Program in Montepllier

 

 

An International PhD Program was recently created by the Doctoral School «CBS2-Sciences Chimiques et Biologiques pour la Santé » to attract students who wish to undertake a thesis financed by french government. In 2009, 18 PhD grants will be attributed. General information about Program statutes, students selection, associated research groups and online application form are available on the website : http://ecole-doctorale-cbs2.igh.cnrs.fr/

Warning : the deadline for online application to the Program is march, 30th 2009

Quantitative analysis of catabolic regulation in single bacterial live cells and complex solutions using fluorescence fluctuation spectroscopy and imaging

Contact for this proposal: Dr. Nathalie Declerck or Cathy Royer, Centre de Biochimie Structurale, CNRS-INSERM, 29 rue de Navacelles, 34090 Montpellier France
nathalie@cbs.cnrs.fr, royer@cbs.cnrs.fr
 
This thesis project aims at the quantitative characterization of a model biological system, the regulation of the central carbon metabolism (CCM) in Gram positive bacteria using state-of-the-art biophotonics methodologies on single molecules based primarily on fluorescence fluctuation spectroscopy (FFS), both in vivo and in vitro. The results obtained in single live cells will be compared with those obtained in solution and crowded media, providing insights into the missing link between in vitro characterization of transcription factors and their functioning in the cellular environment. These results will be interpreted in the context of structure-function and systems biology studies within a long-term collaborative project between the Centre de Biochimie Structurale (CBS in Montpellier) and the Laboratoire de Microbiologie et Génétique Moléculaire (at INRA AgroParisTech). 
The student will use two-photon two-colour fluorescence fluctuation methods on the setup now available at the CBS. With this instrument, fluorescent correlation and cross-correlation spectroscopy (FCS and FCCS) measurements have already been performed for in vitro characterization of different interacting macromolecules, as well as live-cell quantification of the conditional interaction of human nuclear receptors with co-regulators. In addition to classical fluctuation measurement, we will implement other innovative fluorescence fluctuation spectroscopy and imaging techniques, in particular scanning FCS (sFCS), Raster Scanning Image Correlation Spectroscopy (RICS), Number and Brightness Analysis (NBA) and PhotoActivatible Localization Microscopy (PALM).
In the context of these studies the PhD student will study in particular, the interactions between diffusing transcription factors implicated in CCM regulation in Bacillus subtilis using two color, two photon fluorescence cross-correlation spectroscopy in live cells. The dynamics and stochastic nature of the cellular response will be investigated in single bacterial cells under different growth conditions and carbon sources. Preliminary data on GFP reporter expression from CCM promoters in B. subtilis using two photon FCS has already demonstrate the feasibility of such studies in vivo. Encouraging results have also already been obtained in vitro in complex solutions suggesting that molecular crowding significantly modifies the interactions of transcription factors with DNA and effector ligands. Thus, this thesis project is based on very strong preliminary results.

Nathalie DECLERCK

Centre de Biochimie Structurale
INSERM U554/CNRS 5048
29 rue de Navacelles
F-34090 Montpellier, France
Tel: 33 4 67 41 79 11
Fax: 33 4 67 41 79 13
e-mail: nathalie@cbs.cnrs.fr

PhD Program in Montepllier
>
>
>An International PhD Program was recently created by the Doctoral School
>«CBS2-Sciences Chimiques et Biologiques pour la Santé » to attract
>students who wish to undertake a thesis financed by french government. In
>2009, 18 PhD grants will be attributed. General information about Program
>statutes, students selection, associated research groups and online
>application form are available on the website :
>http://ecole-doctorale-cbs2.igh.cnrs.fr/
>
>Warning : the deadline for online application to the Program is march,
>30th 2009
>
>Quantitative analysis of catabolic regulation in single bacterial live
>cells and complex solutions using fluorescence fluctuation spectroscopy
>and imaging
>
>Contact for this proposal: Dr. Nathalie Declerck or Cathy Royer, Centre de
>Biochimie Structurale, CNRS-INSERM, 29 rue de Navacelles, 34090
>Montpellier France
>nathalie@cbs.cnrs.fr, royer@cbs.cnrs.fr
>
>This thesis project aims at the quantitative characterization of a model
>biological system, the regulation of the central carbon metabolism (CCM)
>in Gram positive bacteria using state-of-the-art biophotonics
>methodologies on single molecules based primarily on fluorescence
>fluctuation spectroscopy (FFS), both in vivo and in vitro. The results
>obtained in single live cells will be compared with those obtained in
>solution and crowded media, providing insights into the missing link
>between in vitro characterization of transcription factors and their
>functioning in the cellular environment. These results will be interpreted
>in the context of structure-function and systems biology studies within a
>long-term collaborative project between the Centre de Biochimie
>Structurale (CBS in Montpellier) and the Laboratoire de Microbiologie et
>Génétique Moléculaire (at INRA AgroParisTech).
>The student will use two-photon two-colour fluorescence fluctuation
>methods on the setup now available at the CBS. With this instrument,
>fluorescent correlation and cross-correlation spectroscopy (FCS and FCCS)
>measurements have already been performed for in vitro characterization of
>different interacting macromolecules, as well as live-cell quantification
>of the conditional interaction of human nuclear receptors with
>co-regulators. In addition to classical fluctuation measurement, we will
>implement other innovative fluorescence fluctuation spectroscopy and
>imaging techniques, in particular scanning FCS (sFCS), Raster Scanning
>Image Correlation Spectroscopy (RICS), Number and Brightness Analysis
>(NBA) and PhotoActivatible Localization Microscopy (PALM).
>In the context of these studies the PhD student will study in particular,
>the interactions between diffusing transcription factors implicated in CCM
>regulation in Bacillus subtilis using two color, two photon fluorescence
>cross-correlation spectroscopy in live cells. The dynamics and stochastic
>nature of the cellular response will be investigated in single bacterial
>cells under different growth conditions and carbon sources. Preliminary
>data on GFP reporter expression from CCM promoters in B. subtilis using
>two photon FCS has already demonstrate the feasibility of such studies in
>vivo. Encouraging results have also already been obtained in vitro in
>complex solutions suggesting that molecular crowding significantly
>modifies the interactions of transcription factors with DNA and effector
>ligands. Thus, this thesis project is based on very strong preliminary
>results.
>
>
>
>
>
>Nathalie DECLERCK
>
>Centre de Biochimie Structurale
>INSERM U554/CNRS 5048
>29 rue de Navacelles
>F-34090 Montpellier, France
>Tel: 33 4 67 41 79 11
>Fax: 33 4 67 41 79 13
>e-mail: nathalie@cbs.cnrs.fr
>
>
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